molecular characterization and determination of the antibacterial activity of bacterial endophytes from ocimum sanctum linn lamiaceae - IIP Series

Publication Type: Edited Book

MOLECULAR CHARACTERIZATION AND DETERMINATION OF THE ANTIBACTERIAL ACTIVITY OF BACTERIAL ENDOPHYTES FROM OCIMUM SANCTUM LINN. (LAMIACEAE)

Book Name: Recent Trends in Endophyte Research V4B34
Authors: Sareen Sarah John, Aiswarya P Hariharan
Keywords: MOLECULAR CHARACTERIZATION AND DETERMINATION OF THE ANTIBACTERIAL ACTIVITY OF BACTERIAL ENDOPHYTES FROM OCIMUM SANCTUM LINN. (LAMIACEAE)
Area/Stream: BIOTECHNOLOGY, GENOMICS & BIOENGINEERING / Other /
Published in: IIP Series
Volume: 4, Month:May,Year:2024
Page No.: 221-231
e-ISBN: 978-93-6252-954-1
DOI: https://www.doi.org/10.58532/nbennurch275

Abstract:

Endophytic microorganisms of medicinal plants have gained great importance, and new evidence points to their significant ecological and biotechnological importance. Endophytes are indeed a group of organisms, such as fungi, bacteria, or actinomycete, which live in plant tissues. Although many endophytes have mutually beneficial relationships with plants, not all endophytes are symbiotic. Some endophytes can damage plants under certain conditions and cause more harm instead of providing benefits. Antimicrobial activity is attributed to the presence of endophytes in the plant. This study focuses on the isolation, characterization, and screening of endophytes associated with Ocimum sanctum leaves and stem. The aim is to evaluate the antibacterial properties of the endophytic bacteria. Genomic DNA extraction, PCR analysis, and sequence analysis were performed to characterize the molecular features of the isolated endophytes. Research results confirm the use of Ocimum sanctum against pathogenic microorganisms. It also highlights the importance of isolating and identifying antimicrobials from endophytic bacteria as a valuable approach in the search for new natural products. A total of 30 endophytic species were isolated from the leaves and stem of Ocimum sanctum and screened for their antimicrobial activity using antibiotic discs containing Thrombomycin, Chloramphenicol, and Streptomycin. Additionally it was also determined that these species produce enzymes such as cellulase, amylase, pectinase, and protease. Overall, this study demonstrates the potential of endophytic bacteria associated with aromatic shrubs such as Ocimum sanctum. Its use as a source of antimicrobial emphasizes the importance of further research in this area.

Cite this: Sareen Sarah John, Aiswarya P Hariharan, MOLECULAR CHARACTERIZATION AND DETERMINATION OF THE ANTIBACTERIAL ACTIVITY OF BACTERIAL ENDOPHYTES FROM OCIMUM SANCTUM LINN. (LAMIACEAE), In: Recent Trends in Endophyte Research V4B34, IIP Series, Volume 4, May, 2024, pp. 221-231, Iterative International Publishers IIP, E-ISBN: 978-93-6252-954-1, DOI: https://www.doi.org/10.58532/nbennurch275

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